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Comparative 16S Metabarcoding of Nile Tilapia Gut Microbiota from the Northern Lakes of Egypt

Nile tilapia, Oreochromis niloticus, is the principal fish bred in Egypt. A pilot study was designed to analyze the bacterial composition of the Nile tilapia fish guts from two saltwater lakes in Northern Egypt. Fish samples were obtained from two Delta lakes: Manzala (ML) and Borollus (BL). DNA was extracted, and the bacterial communities in the stomach content were classified (down to the species level) using the 16S rRNA-based analysis. From the two metagenomics libraries in this study, 1,426,740 reads of the amplicon sequence corresponding to 508 total taxonomic operational units were

Healthcare

Computing the burrows-wheeler transform of a string and its reverse

The contribution of this paper is twofold. First, we provide new theoretical insights into the relationship between a string and its reverse: If the Burrows-Wheeler transform (BWT) of a string has been computed by sorting its suffixes, then the BWT and the longest common prefix array of the reverse string can be derived from it without suffix sorting. Furthermore, we show that the longest common prefix arrays of a string and its reverse are permutations of each other. Second, we provide a parallel algorithm that, given the BWT of a string, computes the BWT of its reverse much faster than all

Artificial Intelligence
Healthcare
Software and Communications

An automatic gene ontology software tool for bicluster and cluster comparisons

We propose an Automatic Gene Ontology (AGO) software as a flexible, open-source Matlab software tool that allows the user to easily compare the results of the bicluster and cluster methods. This software provides several methods to differentiate and compare the results of candidate algorithms. The results reveal that bicluster/cluster algorithms could be considered as integrated modules to recover the interesting patterns in the microarray datasets. The further application of AGO could to solve the dimensionality reduction of the gene regulatory networks. Availability: AGO and help file is

Artificial Intelligence
Healthcare

Fast fractal modeling of mammograms for microcalcifications detection

Clusters of microcalcifications in mammograms are an important early sign of breast cancer in women. Comparing with microcalcifications, the breast background tissues have high local self-similarity, which is the basic property of fractal objects. A fast fractal modeling method of mammograms for detecting the presence of microcalcifications is proposed in this paper. The conventional fractal modeling method consumes too much computation time. In the proposed method, the image is divided into shade (homogeneous) and non-shade blocks based on the dynamic range and only the non-shade blocks are

Artificial Intelligence
Healthcare

BicATPlus: An automatic comparative tool for Bi/Clustering of gene expression data obtained using microarrays

In the last few years the gene expression microarray technology has become a central tool in the field of functional genomics in which the expression levels of thousands of genes in a biological sample are determined in a single experiment. Several clustering and biclustering methods have been introduced to analyze the gene expression data by identifying the similar patterns and grouping genes into subsets that share biological significance. However, it is not clear how the different methods compare with each other with respect to the biological relevance of the biclusters and clusters as well

Artificial Intelligence
Healthcare

Maximum likelihood estimator for signal intensity in STEAM-based MR imaging techniques

Stimulated echo acquisition mode (STEAM) is a generic imaging technique that lies at the core of many magnetic resonance imaging (MRI) techniques such MRI tagging, displacement encoded MRI, black-blood cardiac imaging. Nevertheless, tissue deformation causes frequency shift of the MR signal and leads to severe signal attenuation. In this work, a maximum likelihood estimator for the signal amplitude is proposed and used to correct the image artifacts. Numerical simulation and real MR data are used to test and validate the proposed method. © 2011 IEEE.

Artificial Intelligence
Healthcare

Ambient and wearable sensing for gait classification in pervasive healthcare environments

Pervasive healthcare environments provide an effective solution for monitoring the wellbeing of the elderly where the general trend of an increasingly ageing population has placed significant burdens on current healthcare systems. An important pervasive healthcare system functionality is patient motion analysis where gait information can be used to detect walking behavior abnormalities that may indicate the onset of adverse health problems, for quantifying post-operative recovery, and to observe the progression of neurodegenerative diseases. The development of accurate motion analysis models

Artificial Intelligence
Healthcare
Software and Communications

AmpliconNet: Sequence Based Multi-layer Perceptron for Amplicon Read Classification Using Real-time Data Augmentation

Taxonomic assignment is the core of targeted metagenomics approaches that aims to assign sequencing reads to their corresponding taxonomy. Sequence similarity searching and machine learning (ML) are two commonly used approaches for taxonomic assignment based on the 16S rRNA. Similarity based approaches require high computation resources, while ML approaches dont need these resources in prediction. The majority of these ML approaches depend on k-mer frequency rather than direct sequence, which leads to low accuracy on short reads as k-mer frequency doesnt consider k-mer position. Moreover

Artificial Intelligence
Healthcare

Benchmarking of Antimicrobial Resistance Gene Detection Tools in Assembled Bacterial Whole Genomes

Antimicrobial resistance (AMR) is one of the ten dangers threatening our world, according to the world health organization (WHO). Nowadays, there are plenty of electronic microbial genomics and metagenomics data records that represent host-associated microbiomes. These data introduce new insights and a comprehensive understanding of the current antibiotic resistance threats and the upcoming resistance outbreak. Many bioinformatics tools have been developed to detect the AMR genes based on different annotated databases of bacterial whole genome sequences (WGS). The number and structure of

Artificial Intelligence
Healthcare

Strain correction in interleaved strain-encoded (SENC) cardiac MR

The strain encoding (SENC) technique directly encodes regional strain of the heart into the acquired MR images and produces two images with two different tunings so that longitudinal strain, on the short-axis view, or circumferential strain on the long-axis view, are measured. Interleaving acquisition is used to shorten the acquisition time of the two tuned images by 50%, but it suffers from errors in the strain calculations due to inter-tunings motion of the heart. In this work, we propose a method to correct for the inter-tunings motion by estimating the motion-induced shift in the spatial

Artificial Intelligence
Healthcare
Software and Communications
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